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- ConfocalMicroscopy + (Confocal microscopy, most frequently confo … Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation.ock out-of-focus light in image formation.)